Sclerotization of Insect Cuticle in a Cell-Free System
نویسندگان
چکیده
Incubation o f deproteinized larval cuticle (chitin flakes) with purified arylphorin (calliphorin) or larval haemolymph o f Calliphora vicina resulted in the formation o f a chitin-protein complex. Enzymatic oxidation o f N-ß-alanyldopamine (N D A B ) in the presence o f chitin flakes or the chitin-protein complex, resulted in various degrees o f cross-linking o f N BA D quinone formed with chitin. This study confirms the involvement o f arylphorin in the process o f quinone tanning o f insect cuticle.
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Model reactions for insect cuticle sclerotization: participation of amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36.
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